5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. Description 1. Spectramax M5. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. 1. Standard features include a cuvette port, spectral scanning in 1 nm increments, and. The chamber. This reader's dual monochromators for. 2. SpectraMax M5e Multi-Mode Microplate Reader. Description. ) Open SoftMax Pro, set Preference to SpectraMax L, and try the different available COM ports to find the one for the SpectraMax L. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. FlexStation 3 reader Fluorescence Microplate Reader Comparison Chart . #655801. The chamber. 0% ± 0. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. What plates do you recommend for SpectraMax Plate Readers? Luminescence : Solid white plates for top reads and white plates with clear bottoms for. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. com 4. Manufacturer: Molecular Devices. spr SpectraMax iD3 SpectraTest ABS2 v1. plate type, read area and number of images per well, plus specify positive/negative wells. In this. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orMolecular Devices Spectramax M5 Plate Reader is available from The Lab World Group. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. The guide also covers the software features, the specifications, and the accessories of the systems. book Page 1 Wednesday, July 7, 2010 2:09 PMThe Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. Be sure to insert . We would like to show you a description here but the site won’t allow us. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. SpectraTest ABS1 Absorbance Plates, FL1 Fluorescence Plates, and LM1 Luminescence Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. M5 and M5e Multi-Mode Microplate Readers User Guide SpectraMax M3, M4, M5. Pathcheck Pathlength Measurement Technology. Comes from Lab closure Approx. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. youusehighprofile(6-well,12-well,24-well,or48-well)plates,youmustremovethe plateadapterfromtheplatedrawer. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a. After the plate is read, data will appear in the plate. 312 gain calibration failed. When the user applies a set temperature of 37°C, the top is heated to 37. 000000 out of 5. Label: provides information. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. * BD Falcon® Microtest™ plates #353075 and # 353072). The SpectraMax M2 and M2e are versatile and reliable. Choose from a three- (M3), four- (M4), orThe SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Among the various ready-to-read protocols, users will find pre-written SpectraDrop Plate protocols enabling Micro Volume applications measurement. FlexStation II 384. Molecular devices SpectraMax M5e Pdf User Manuals. The drawing shows a standard microplate of 15 mm height covered with a lid. readers, the vertical light beam results in a pathlength that depends on the vol-. 4. Shop all used microplate readers and parts from a variety of manufacturers. Turn the reader on and secure the USB cable. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. At NanoRacks, the challenge for the engineers will be to modify the plate reader in order for the unit to operate comfortably in the zero-gravity environment of the space station. In an analogous manner, the same sample names were assigned to a CuvetteSet section. Microplate Readers. UV/Vis Photometric Performance: Wavelength Range. 9. Optical absorbance was measured at 734 nm on a SpectraMax M5. 7°C (gradient heating design). A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. 00. Trends in Immunology. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck®. SeePlateAdapteronpage20. Third this recommendation. 7°C (gradient heating design). See SpectraMax® Microplate Reader User Manual Download Page. SpectraMax M5 Multi-Mode Microplate Reader. Shop now. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. ". l. This process should take about 3 - 5 minutes. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The SpectraMax Plus 384 Microplate Reader for absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. PURPOSE. *This plate definition in SoftMax Pro Software is the same for black and white plates. Set up a plate section for a second emission scan as in Step C above, except for the added Emission Cutoff Filter. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. 3 or greater. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. Description The Onboard Microprocessor. Note: Check the Applies to section at the bottom of the page to make this is the proper driver for your plate reader. 010 OD 384-Well Plate,. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. 0397 in x 3. For more information please visit us at: M 系列多功能微孔板读板机包括 M2/M2e、M3、M4、M5/M5e 等几款多功能酶标仪。. Five preferred detection modes. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. When the user applies a set temperature of 37°C, the top is heated to 37. 6 for further experiment, so I'm confused which i would choose for OD value determination. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. 40266 Email: [email protected] only) SpectraMax M-series Microplate readers. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. The plate is set up for reading and images are acquired according to specified parameters. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. 0200-0729. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Category 2 and 3 microplate readers use gradient heating as well. M3-M5e_manual. Features. 2. The SpectraMax M5 e reader has the same performance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF. Opens in a new window or tab. What do I do now? In our tests, the Greiner Bio-One Fluotrac™ 200 Microplate has shown lower background. Molecular Devices. 5 pM (0. 2. 2. Description 1. Table 1. e. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. SpectraMax iD5 Multi-Mode Microplate Reader. Plate section without PathCheck applied. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. 18 x 14 x 8 ins. 0 . MICROPLATE READER: Used, in good condition, working microplate reader. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and wester. 标准功. Plate reader instructions Computer. HTRF-compatible SpectraMax Multi-Mode Readers. Following treatment the samples were assayed for protease activity in triplicate using the Pierce Fluorescent Protease Assay Kit (Thermo Scientific, Waltham USA),. Molecular Devices SpectraMax Plus 384. SpectraMax M5 Plate Reader SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. The following document is. 87. Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Spectramax M5. Bookings are. ”The SpectraMax 190 reader can be integrated with Molecular . Examples: “Press carefully to engage,” “ Do not press down. We carry both single-mode and multi-mode readers from manufacturers such as BioTek Instruments, Perkin Elmer, and SRU Biosystems. This microplate reader handles Absorbance, Fluorescence Intensity, luminescence, Time resolved fluorescence, fluorescence polarization. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A 5 1. 8. To update the driver. Clear Filters. OPTICS Mirrored optics focus the light into the sample volume, and cutoff filters are used to. 4. Molecular Devices microplate readers. . Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. Choose from a three- (M3), four-. both cuvette-based and microplate. The substrate solution (100 μL/well) was subsequently employed for 5-min reaction and the absorbance at 370 nm was monitored on a SpectraMax M5 plate reader (Molecular Devices). Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. Instructions on the proper use of the SpectraMax M5 Plate Reader. The BD plate dimensions are 5. Multimode Readers Comparison Chart. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Only Molecular Devices microplate plate readers offer the capability to . Bioz Stars score: 86/100, based on 1 PubMed citations. 00800 665 32860. Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The SpectraMax® ABS Plus Microplate Reader can run . The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The M5e Reader is easily automated. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Some familiarity with Windows should be sufficient to use it. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug. Accordingly there are different configurations possible. Microplate readers’ standard detection modes. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. For example, microplates containing different samp les can be read using the same orThe Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The SpectraMax L is now connected to SoftMax Pro sofware. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. series measured in a 96-well plate in the SpectraMax M5 and the Analyst GT. 9. SpectraMax i3x Multi-Mode Microplate Re. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Reader Documentation Version No. After the plate is read, data will appear in the plate section: 11. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. edu, 814-863-3503. edu, 814-863-3503. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. Thermo Scientific Microplate Reader Verification Plates Cat# 24072800. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. The performance of these two modes is not comparable to dedicated luminescence or timeresolved fluorescence instruments, or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. Both full length human PI4KIIIB and the truncated PI4KIIIB crystal construct with the c-term were run at 200 nM. free tracer decreases, resulting in an. It also integrates with the. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96-well black plates. GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. These lab workhorses are used in drug discovery. . : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. com 10. A solution for low volume applications. Fluorescein 0. After the plate is read, data will appear in the plate. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). 0 out of 5 $ 3,799. . REF - For the SpectraMax Plus 384, press to do a Cuvette Reference correction method read of buffer, water, or air taken in the cuvette that is used as I to calculate Absorbance or % Transmittance with the PathCheck Pathlength Measurement Technology. Molecular Devices SpectraMax M2 Multimode Plate Reader. SpectraMax Paradigm Multi-Mode Safety Guide SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. 04 to 3. While they play an important role in generating data from a wide range of. Choose cables that meet the requirement described in this appendix. : Please ensure that the plate dimensions entered in the SoftMax Pro software Acquisition Settings (Read Settings > Plate Type > Edit) correspond to those on the specific microplate data sheet, provided by the plate manufacturer. The plate reader can measure multiple readouts, including UV and visible absorbance. • Tel. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all your future lab needs. SpectraMax L reader, SpectraMax M5/M5e reader What is the StakMax timing with SpectraMax Plate Readers? If using the StakMax and a SpectraMax reader together, the time specifications for the movement of the StakMax are as follows:. Select Update Driver. $6,649. Gemini XPS. The instrument settings were identical to those used in Example 1 above. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. 306 plate air cal fail,low light. In this application note, we describe how the SpectraMax® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. spr SpectraMax iD5 SpectraTest ABS2 v1. 0 . Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. A standard curve was plotted using a 4-parametric. . SpectraMax® M5 Multi-Mode Microplate Re. To assess the sensitivity of each microplate reader, a plate was set up with 32 wellsThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. After the plate is read, data will appear in the plate section: 11. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Shake. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. 4. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. View online or download Molecular devices SpectraMax M5 User Manual. For a higher . 1. Read More. Fluorescence Microplate Reader Comparison Chart. When a feature or capability applies to only certain readers, this exception is noted. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. SpectraMax® M5 Microplate Reader Molecular Devices LLC Applications: Absorbance,. An integration time of 1 second was used for each reader. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic Gq-coupled receptor. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or We use Spectramax M5 microplate reader in our lab. Wide range of applications The VersaMax reader covers a wide range of applications:Molecular Devices. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. View the Molecular Devices website. Our lab regularly left plates with aqueous buffer in them in the plate reader (service contract lapsed for several years. The M5 can read volumes down to 2uL with the uMax Low Volume Plate. Choose from a three- (M3), four- (M4), orProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. HTRF-compatible SpectraMax Multi-Mode Readers. Equipped with scanning, shaking and kinetics modules. For prep samples treated with the ELISA kit reagents, it is fast and user friendly. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with abs. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the. 8. Call 516-939-0888 to check. In the M2, M5 and Plus. spr * SpectraMax i3(x) SpectraTest ABS2 v1. ELISA Plate Readers / Microplate Readers. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was. 1. We empower scientists to advance discovery Automated, end-to-end solutions span research disciplines to help scientists discover, scale, and industrialize biology. 4. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Page 1 of 8 . Spectramax Softmax Pro 6. 3) At the bottom of the strip, click on "Show. For SpectraMax L or LMax II, LMax II. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. 40266 Email: [email protected] OD ± 3. 40266 Email: drugdiscoverytech@lifetech. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. SpectraMax M5. Be sure to insert the purple (SpectraMax M5 or M5e reader) or black (FlexStation 3 reader) plate adapter in the plate carriage prior to Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. M5 E -HTRF Readers. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. SpectraMax Injector Cartridge with SmartInject™ Technology. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays. easy to use and read multiple plates very quickly. SpectraMax ABS Plus SpectraTest ABS2 v1. The chamber. Dynamic Range. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. 0 out of 5 $ 15,999. 1. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. These robust readers have been placed in labs from. Molecular Devices. 03 in x 3. In the lower half of the window, left side, there is a box labeled reader. An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 2. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. The Analyst GT is equipped with 20 plate magazines in a bi-directional stacker. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. ScanLater Western Blot Detection Cartridge. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. PURPOSE. 3752 in as per SBS dimensions. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. There is currently no charge for using this plate reader. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter Created Date:1) Select the correct plate and the wells that you want to measure. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. the purple (SpectraMax M5 or M5e reader) or black (FlexStation 3 reader) plate adapter in the plate carriage prior to. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. Serial number location on Series II and Multi-Mode plate readers. Devices’ StakMax® Microplate Stacker in a matter of minutes and . : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The SpectraMax iD5 reader rapidly performs absorbance measurements that are used to plot standard curves and calculate sample concentrations using a preconfigured protocol in SoftMax Pro Software. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). SpectraMax® 340PC384 Microplate Reader for acquiring data in endpoint, kinetic, and spectral scan modes using wavelegths from 340-850 nm, tunable in 1 nm increments.